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Last updated: May 2026
Fort Caroline was an attempted French colonial settlement in Florida, located on the banks of the St. Johns River in present-day Duval County. It was established under the leadership of René Goulaine de Laudonnière on June 22, 1564, as a new territorial claim in French Florida and a safe haven for Huguenots, who were being persecuted in France because they were Protestant, rather than Catholic. The French colony came into conflict with the Spanish, who established St. Augustine in September 1565, and Fort Caroline was sacked by Spanish troops under Pedro Menéndez de Avilés on September 20. The Spanish continued to occupy the site as San Mateo until 1569. The exact site of the former fort is unknown. In 1953 the National Park Service established the Fort Caroline National Memorial along the southern bank of the St. John's River near the point that commemorates Laudonnière's first landing. This is generally accepted by scholars as being in the vicinity of the original fort, though probably not the exact location. The memorial is now managed as a part of the Timucuan Ecological and Historic Preserve, but it is also a distinct unit under administration of the National Park Service.
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